ACE (rs4646994) and MTHFR (rs1801133) single nucleotide polymorphisms in type 2 Diabetes Mellitus patients with dyslipidemia

  • Nervana Bayoumy Department of Physiology, College of Medicine - King Saud University, Riyadh, Kingdom of Saudi Arabia
  • Mohammed Mohamed El-Shabrawi Department of Clinical and Chemical Pathology, Faculty of Medicine - Suez Canal University, Ismailia, Egypt
  • Ola Farouk Leheta Department of Clinical and Chemical Pathology, Faculty of Medicine - Suez Canal University, Ismailia, Egypt
  • Hamdy Hassan Omar Department of Internal Medicine, Faculty of Medicine - Suez Canal University, Ismailia, Egypt
Keywords: ACE, MTHFR, Single Nucleotide Polymorphisms, Diabetes mellitus

Abstract

Dyslipidemia associated with diabetes mellitus (DM) is the most common cause of coronary artery disease mortality. Both genetic and environmental factors have a role in the development of type 2 DM and dyslipidemia. The current study aims to find the role of angiotensin-converting enzyme (ACE) (rs4646994) and methyl-tetra-hydro-folate reductase (MTHFR) (rs1801133) polymorphisms with dyslipidemia in type 2 DM patients. One hundred and fifty (150) type 2 DM patients with dyslipidemia were included in this study, in addition to 150 type 2 DM patients without dyslipidemia and 150 healthy age and sex-matched control group. Venous blood samples were collected for the different laboratory workups and the gene polymorphism testing using the polymerase chain reaction-restriction enzyme fragment polymorphism (PCR-RFLP) technique. Both demographic and clinical parameters were collected from the study population. The results showed significant associations between ACE and MTHFR polymorphisms among the two patient groups (type 2 DM with dyslipidemia, with p-value<0.001 and 0.011, respectively), (type 2 DM without dyslipidemia with p-value=0.007 and 0.016, respectively). This study supports the notion that these commonly found ACE and MTHFR polymorphisms could be incriminated in the pathogenesis of dyslipidemia associated with type 2 DM.

Published
2022-11-25
How to Cite
Bayoumy, Nervana, Mohammed Mohamed El-Shabrawi, Ola Farouk Leheta, and Hamdy Hassan Omar. 2022. “ACE (rs4646994) and MTHFR (rs1801133) Single Nucleotide Polymorphisms in Type 2 Diabetes Mellitus Patients With Dyslipidemia”. Romanian Journal of Diabetes Nutrition and Metabolic Diseases 29 (4), 401-6. http://rjdnmd.org/index.php/RJDNMD/article/view/1070.