The role of Indonesianin-5 in HMG CoA reductase and sterol regulatory element binding protein-2 expressions of hypercholesterolemic rat

  • Noviyanty Indjar Gama Faculty of Pharmacy, Mulawarman University, Samarinda, Indonesia
  • Prasetyastuti Prasetyastuti Department of Biochemistry, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
  • Sunarti Sunarti Department of Biochemistry, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Keywords: Indonesianin-5, SREBP-2, HMG CoA reductase, RT-PCR, anti-hypercholesterolemia

Abstract

Increased cholesterol level increases the risk of heart disease and stroke. Sterol Regulatory Element Binding Protein-2 (SREBP-2) is a transcription factor for the synthesis of cholesterol genes. SREBP-2 can induce the expression of HMG CoA Reductase (HMGR). HMGR is a key cholesterol synthesis enzyme. Indonesianin-5, derived from Mahogany seeds (Swietenia macrophylla K), is a flavonoid. Indonesianin-5 is known to have a hypocholesterolemic effect. This study aimed to analyze the mechanism of Indonesianin-5 to decrease LDL and cholesterol levels and the expression of hepatic SREBP-2 and HMGR. Rattus norvegicus were grouped into 5 groups: normal group (N), Hypercholesterolemia group (HC), Hypercholesterolemic groups given simvastatin (HC+Sim), 10 mg of Indonesianin-5 (HC+ 10 Ind-5) and 90 mg of Indonesianin-5 (HC+ 90 Ind-5). In silico analysis was used to determine the effects of Indonesianin-5. The expressions of SREBP-2 and HMGR were analyzed by the RT-PCR method. Indonesianin-5 inhibited the activity of the HMGR enzyme. Expression of SREBP-2 was high after induction of Indonesianin-5 (p<0.05). The expression of HMGR was high after induction of Indonesianin-5 (p<0.05). Indonesianis-5 inhibits HMG CoA reductase and increases gene expression of SREBP-2 and HMG CoA reductase.

Published
2023-09-30
How to Cite
Gama, Noviyanty, Prasetyastuti Prasetyastuti, and Sunarti Sunarti. 2023. “The Role of Indonesianin-5 in HMG CoA Reductase and Sterol Regulatory Element Binding Protein-2 Expressions of Hypercholesterolemic Rat”. Romanian Journal of Diabetes Nutrition and Metabolic Diseases 30 (3), 282-91. https://rjdnmd.org/index.php/RJDNMD/article/view/1064.