ACE (rs4646994) and MTHFR (rs1801133) single nucleotide polymorphisms in type 2 Diabetes Mellitus patients with dyslipidemia
Dyslipidemia associated with diabetes mellitus (DM) is the most common cause of coronary artery disease mortality. Both genetic and environmental factors have a role in the development of type 2 DM and dyslipidemia. The current study aims to find the role of angiotensin-converting enzyme (ACE) (rs4646994) and methyl-tetra-hydro-folate reductase (MTHFR) (rs1801133) polymorphisms with dyslipidemia in type 2 DM patients. One hundred and fifty (150) type 2 DM patients with dyslipidemia were included in this study, in addition to 150 type 2 DM patients without dyslipidemia and 150 healthy age and sex-matched control group. Venous blood samples were collected for the different laboratory workups and the gene polymorphism testing using the polymerase chain reaction-restriction enzyme fragment polymorphism (PCR-RFLP) technique. Both demographic and clinical parameters were collected from the study population. The results showed significant associations between ACE and MTHFR polymorphisms among the two patient groups (type 2 DM with dyslipidemia, with p-value<0.001 and 0.011, respectively), (type 2 DM without dyslipidemia with p-value=0.007 and 0.016, respectively). This study supports the notion that these commonly found ACE and MTHFR polymorphisms could be incriminated in the pathogenesis of dyslipidemia associated with type 2 DM.