Does TP53 gene polymorphism increase the risk of obesity and chronic pancreatitis comorbidities in type 2 diabetic patients?
Background and aims: to establish the prevalence of genotype frequencies for TP53 (rs1042522) alleles in type 2 diabetic patients with comorbid overweight/obesity and chronic pancreatitis (CP). Material and methods: The study involved 34 type 2 diabetic patients and 10 healthy individuals. Genomic DNA was extracted from peripheral blood leukocytes using a commercially available DNA isolation kit. The TP53 gene rs1042522 C>G polymorphism was genotyped using the Taq Man real-time PCR method. Results: We found a significant association between the C/C and C/G genotypes of TP53 rs1042522 SNP polymorphism in type 2 diabetic patients with normal body weight without CP and in type 2 diabetic patients with overweight/obesity. However, no significant difference was found between genotypes С/С, C/G and G/G of rs1042522 SNP in TP53 gene in type 2 diabetic patients with obesity and CP vs control group. Conclusions: The presence of the C allele of TP53 SNP rs1042522 polymorphism in both homozygous and heterozygous states may indicate the increased risk of comorbid obesity development in type 2 diabetic population of Ternopil region, Ukraine. Small number of patients used in this study warrants further research.