Effect of fermented milk Lactiplantibacillus pentosus HBUAS53657 on blood glucose, lipid profiles and inflammation in high-fat diet-induced mice
Lactobacillus pentosus strains with health-promoting properties, such as immunomodulatory and antiproliferative activities, are potential probiotic strains. L. pentosus HBUAS53657 is one of the most common strains found in dadih (buffalo fermented milk) originating from Tanjung Bonai Lintau Buo Utara. A previous study found that L. pentosus HBUAS53657 had probiotic characteristics. It is necessary to investigate the effect of L. pentosus HBUAS53657 fermented milk probiotics on inflammatory markers in experimental animals. This study is a true experimental design with only a post-test control group. The population consisted of Wistar rats (Rattus norvegicus) n=28 divided into four groups: the control group (ND), the High Fat Diet (HFD) group, the High Fat Diet and L. pentosus HBUAS53657 group 109 CFU/ml (HFDL), and the High Fat Diet and fermented milk with L. pentosus strain HBUAS53657 109 CFU/ml and 20% oranges group (HFDSF). This feeding was done for six weeks (42 days). The research data was processed and statistically analyzed using the Way ANOVA test, followed by the Post hoc test. The results showed that L. pentosus HBUAS53657 fermented milk slowed weight gain in rats given HFDSF by 15.36%, compared to 18.26% in the HFDL group and 24.30% in the HFD group (P<0.01). When compared to the HFD group, the HFDSF and HFDL groups had lower blood glucose, total cholesterol, triglycerides, and LDL (p<0.05, p<0.01, P<0.05). L. pentosus HBUAS53657 reduced the level of serum TNF-α on HFD but not significantly. This demonstrates the protective effect of L. pentosus HBUAS53657 on lipid metabolism disorders in rats fed high-fat rats. L. pentosus HBUAS53657 can reduce HFD-induced proinflammatory cytokines.