Assessment of adipose tissue hormone levels after sleeve gastrectomy in rats with experimental metabolic syndrome

Myroslav Kritsak; Serhii Konovalenko; Iryna Stechyshyn; Bohdana Pavliuk; Tetiana Gargula; Viktor Shatskyi

Myroslav Kritsak Department of Surgery, Faculty of Postgraduate Education, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
Serhii Konovalenko Department of Operative Surgery and Clinical Anatomy, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
Iryna Stechyshyn Department of Pharmacy Management, Economics and Technology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
Bohdana Pavliuk Department of Pharmacy Management, Economics and Technology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
Tetiana Gargula Department of Operative Surgery and Clinical Anatomy, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
Viktor Shatskyi Department of Disaster and Military Medicine, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine

Keywords: insulin resistance, bariatric surgery, leptin, adiponectin, resistin

Abstract

The increase in the number of patients with obesity causes significant interest of researchers and doctors in the study and analysis of physiological and pathological processes in adipose tissue. The aim of the work is to evaluate changes in the level of hormones in adipose tissue after sleeve gastrectomy. Changes in hormone levels were studied in 3 groups of 10 animals each: a control group with a normal weight, a control group with metabolic syndrome, and the main group with metabolic syndrome, which underwent sleeve gastrectomy. Three weeks after sleeve gastrectomy, weight loss was 13.7%, and after 6 weeks – 24.8%. The level of leptin in the control group was 22.08±2.61 ng/g, in the control group with metabolic syndrome – 34.01±3.01 ng/g, and in the main group 6 weeks after surgery, it was 26.92±2, 14 ng/h. When studying the level of adiponectin in the blood of rats, we found a statistically significant difference between the level of the control group with metabolic syndrome, which was 5.16±0.75 μg/ml, and the level in the control group – 7.01±0.87 μg/ml, in the main group 6 weeks after the operation – 6.32±0.57 μg/ml. The level of resistin in the control group was 12.14±1.73 μg/l, in the second group – 17.86±1.91 μg/l, in the main group – 15.06±1.68 μg/l. Sleeve gastrectomy creates hormonal changes that have a positive effect on the main mechanism of reducing the volume of adipose tissue, leading to the normalization of adipokine levels.