Infections with acanthamoeba triangularis in patients with renal failure

  • Mahdi AL-Maryani Technical Institute of Amarah, Southern Technical University, Misan, Iraq
  • Muslim Abdulrahman Mohammed AL-Tooma Department of Pathological Analysis, College of Science, University of Basrah, Basrah, Iraq
Keywords: renal failure, acanthamoeba triangularis, amaranth, acanthopod, trophozoite


This study included 100 samples collected from patients with renal failure from the dialysis centers. NN-agar medium was used as culture, and one sample was infected with the acanthamoeba. It was diagnosed morphologically as Acanthamoeba triangularis. The diagnosis was confirmed by PCR technology, a genetic sequence analyzer and phylogenetic tree analysis based on the 18S ribosomal RNA gene. A total of 100 urine samples from patients with renal failure were collected from the dialysis center and microscopically examined; the result of direct examination of urine sediment was the presence of bacteria. The urine cultivation shows that one case of opportunistic amoeba infection was recorded. It was diagnosed as Acanthamoeba triangularis, and to ensure that the infection was real and not caused by contamination, another sample was taken from the urine of the same patient a month after the first collection and culture. The culture result was similar to the first sample, as the same parasite was isolated. The parasite was mostly triangular; the outer layer contained three open, while the trophozoite stage had many acanthopoda. This is the first record of acanthamoeba triangularis infection in renal failure patients.

How to Cite
AL-Maryani, Mahdi, and Muslim AL-Tooma. 2023. “Infections With Acanthamoeba Triangularis in Patients With Renal Failure”. Romanian Journal of Diabetes Nutrition and Metabolic Diseases 30 (3), 357-63.