Serum levels of 8-hydroxy 2-deoxyguuanosine as a marker of DNA damage in healthy obese individuals

Abdelmarouf Hassan Mohieldein

Abdelmarouf Hassan Mohieldein Associate professor of Medical Biochemistry. Department of Medical Laboratories. College of Applied Medical Sciences. Qassim University

Abstract

Background and aims: Obesity is a serious and growing healthcare concern worldwide. It is associated with mortality and co-morbidity. We aimed to determine serum concentrations of 8-hydroxy-2`-deoxyguanosine and their association with body-mass index in healthy individuals.

Material and method: Fifty-nine apparently healthy individuals were recruited from public places in this cross sectional study. Participants were divided into: obese group and non-obese group. Blood collected; serum and plasma were prepared. Glucose and glycated hemoglobin were assayed by standard methods using commercial kits. 8-hydroxy-2`-deoxyguanosine was determined by enzyme-linked immunosorbent assay. Data were analyzed using software Statistical Packages for the Social Sciences.

Results: Data showed that median (interquartile levels) of serum 8-hydroxy-2`-deoxyguanosine was significantly higher in the obese subjects in comparison with controls. Moreover, a positive correlation was documented between level of serum 8-hydroxy-2`-deoxyguanosine and body-mass index in study participants.

Conclusions: The study findings suggest that weight loss for obese individuals could reduce DNA damage and oxidative stress which underlie the pathogenesis of obesity-associated metabolic disorders.