Serum levels of 8-hydroxy 2-deoxyguuanosine as a marker of DNA damage in healthy obese individuals
Background and aims: Obesity is a serious and growing healthcare concern worldwide. It is associated with mortality and co-morbidity. We aimed to determine serum concentrations of 8-hydroxy-2`-deoxyguanosine and their association with body-mass index in healthy individuals.
Material and method: Fifty-nine apparently healthy individuals were recruited from public places in this cross sectional study. Participants were divided into: obese group and non-obese group. Blood collected; serum and plasma were prepared. Glucose and glycated hemoglobin were assayed by standard methods using commercial kits. 8-hydroxy-2`-deoxyguanosine was determined by enzyme-linked immunosorbent assay. Data were analyzed using software Statistical Packages for the Social Sciences.
Results: Data showed that median (interquartile levels) of serum 8-hydroxy-2`-deoxyguanosine was significantly higher in the obese subjects in comparison with controls. Moreover, a positive correlation was documented between level of serum 8-hydroxy-2`-deoxyguanosine and body-mass index in study participants.
Conclusions: The study findings suggest that weight loss for obese individuals could reduce DNA damage and oxidative stress which underlie the pathogenesis of obesity-associated metabolic disorders.