Serum levels of 8-hydroxy 2-deoxyguuanosine as a marker of DNA damage in healthy obese individuals

  • Abdelmarouf Hassan Mohieldein Associate professor of Medical Biochemistry. Department of Medical Laboratories. College of Applied Medical Sciences. Qassim University

Abstract

Background and aims: Obesity is a serious and growing healthcare concern worldwide. It is associated with mortality and co-morbidity. We aimed to determine serum concentrations of 8-hydroxy-2`-deoxyguanosine and their association with body-mass index in healthy individuals.

Material and method: Fifty-nine apparently healthy individuals were recruited from public places in this cross sectional study. Participants were divided into: obese group and non-obese group. Blood collected; serum and plasma were prepared. Glucose and glycated hemoglobin were assayed by standard methods using commercial kits. 8-hydroxy-2`-deoxyguanosine was determined by enzyme-linked immunosorbent assay. Data were analyzed using software Statistical Packages for the Social Sciences.

Results: Data showed that median (interquartile levels) of serum 8-hydroxy-2`-deoxyguanosine was significantly higher in the obese subjects in comparison with controls. Moreover, a positive correlation was documented between level of serum 8-hydroxy-2`-deoxyguanosine and body-mass index in study participants.

Conclusions: The study findings suggest that weight loss for obese individuals could reduce DNA damage and oxidative stress which underlie the pathogenesis of obesity-associated metabolic disorders.

Published
2020-12-30
How to Cite
Mohieldein, Abdelmarouf Hassan. 2020. “Serum Levels of 8-Hydroxy 2-Deoxyguuanosine As a Marker of DNA Damage in Healthy Obese Individuals”. Romanian Journal of Diabetes Nutrition and Metabolic Diseases 27 (4), 366-71. https://rjdnmd.org/index.php/RJDNMD/article/view/785.