Romanian Journal of Diabetes Nutrition and Metabolic Diseases https://rjdnmd.org/index.php/RJDNMD <p>The <strong>Romanian Journal of Diabetes Nutrition and Metabolic Diseases</strong> is the journal of the <strong>Romanian Society of Diabetes Nutrition and Metabolic Diseases</strong>.</p> <p>It appeared for the first time in 1992, in Romanian language and beginning with 2006 it was published entirely in English. It is a peer reviewed journal, therefore the accepted articles were peer-reviewed by <strong>at least two scientific experts</strong> in the field.</p> <p>It contains <strong>original papers</strong>, including fundamental and clinical research, <strong>literature reviews</strong>, <strong>clinical reports</strong>, <strong>editorials</strong>, articles that contain the <strong>results of the most important clinical studies</strong>, reports from the important congresses and <strong>book reviews</strong>.&nbsp;</p> <p>In Romanian Journal of Diabetes Nutrition and Metabolic Diseases there may be published only papers exclusively sent to this journal, not published previously. The authors should include possible conflicts of interest and financing sources. The laws of copyright and international rules of scientific research are respected.</p> <p>The Romanian Journal of Diabetes Nutrition and Metabolic Diseases <strong>strictly requires</strong> for all the scientific work published <strong>to be 100% compliant</strong> with the&nbsp;<a title="ICMJE.ORG" href="http://www.icmje.org/icmje-recommendations.pdf" target="_blank" rel="noopener">Recommendations for the Conduct, Reporting, Editing, and Publication of Scholarly Work in Medical Journals</a>&nbsp;and with the&nbsp;<a title="Best Practices" href="http://doaj.org/bestpractice" target="_blank" rel="noopener">Principles of Transparency and Best Practice in Scholarly Publishing</a> (joint statement by COPE, DOAJ, WAME, and OASPA).</p> <p>This journal appears trimestrially (four numbers/year). 650-800 copies are printed on each number.</p> <p>ILEX Printing House, Bucharest, has the exclusivity for the typesetting, printing and distribution of the Romanian Journal of Diabetes, Nutrition and Metabolic Diseases.</p> <p>Our journal is recognized by the Romanian National University Research Council (CNCSIS - 2009) to be part in the B+ category, Code 322.</p> <p>The Romanian Journal of Diabetes Nutrition and Metabolic Diseases is currently indexed in:</p> <p>- Index Copernicus<br>- Get Cited<br>- SCOPUS<br>- Scirus<br>- Celdes<br>- CNKI Scholar (China National Knowledge Infrastucture)<br>- CNPIEC<br>- EBSCO - TOC Premier<br>- EBSCO Discovery Service<br>- Google Scholar<br>- J-Gate<br>- Naviga (Softweco)<br>- Primo Central (ExLibris)<br>- SCImago (SJR)<br>- Summon (Serials Solutions/ProQuest)<br>- TDOne (TDNet)<br>- Ulrich's Periodicals Directory/ulrichsweb<br>- WorldCat (OCLC)</p> en-US journal@sanatateapress.ro (Dragoș Crețoiu) journal@sanatateapress.ro (Dragoș Crețoiu) Thu, 23 Jun 2022 20:39:43 +0000 OJS 3.1.1.4 http://blogs.law.harvard.edu/tech/rss 60 Follicular thyroid cancer associated with autoimmune polyglandular syndrome– A case report https://rjdnmd.org/index.php/RJDNMD/article/view/1171 <p><em>Thyroid cancer is the most common malignancy of the endocrine system. We describe the clinical case of 53 years-old Caucasian women with polyglandular autoimmune syndrome diagnosed subsequently with follicular thyroid carcinoma. The patient was diagnosed with type 1 diabetes mellitus in 1997 and Hashimoto thyroiditis in 2000. In 2019 cervical ultrasound highlighted a nodular goiter (left lobular node 29.9/19.9/32.3 mm and small thyroid isthmus nodule 4.5/5.1 mm). Fine needle puncture of the thyroid nodule was indicated but the patient did not perform the procedure. In 2020 a total thyroidectomy was performed. Histopathological examination revealed a micro-angio-invasive capsular follicular carcinoma with oxyphil cellularity on a background of chronic autoimmune thyroiditis. </em></p> <p><em>The association of the two endocrine conditions is controversial but increased insulin levels due to exogenous insulin administration was shown to stimulate (similar to insulin-like growth factor) the follicular cells growth. In addition, chronic hyperglycemia exposure may be involved in the appearance of thyroid cancer by increasing oxidative stress and secondary to activation of the mitogenic pathway. This is the reason why we suggest the careful monitoring of thyroid function and morphology, especially in diabetic women. </em></p> Rucsandra Danciulescu-Miulescu, Loreta Guja, Radu Ilinca ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1171 Thu, 23 Jun 2022 19:05:20 +0000 Seroconversion after Hepatitis B vaccination in chronic kidney disease patients on maintenance hemodialysis: Does diabetes affect seroconversion response? https://rjdnmd.org/index.php/RJDNMD/article/view/1080 <p><strong>Background and aims</strong>: Patients of chronic kidney disease (CKD) on maintenance haemodialysis (MHD) have a higher prevalence of Hepatitis B. Hepatitis B is preventable by vaccination, however the seroconversion response is less effective in patients of CKD. Since diabetes is an important cause of CKD, this study aimed to compare seroconversion rates after Hepatitis B vaccination in normal individuals, patients with non-diabetic CKD (NDCKD) on MHD&nbsp; &nbsp;&nbsp;&amp; patients with diabetic chronic kidney disease (DCKD) on MHD. We also aimed to determine whether an association existed between seroconversion rates &amp; duration of diabetes.</p> <p>&nbsp;</p> <p><strong>Materials &amp; methods</strong>: This was a case control study conducted on the following subjects who had completed the Hepatitis B immunisation schedule - normal subjects, patients with NDCKD stage 5 and patients with DCKD stage 5 on MHD. Anti-HBS levels were measured in the three groups and seroconversion rates were measured &amp; compared.</p> <p>&nbsp;</p> <p><strong>Result:</strong> Of 132 subjects ,44 each were normal controls, NDCKD, and DCKD on MHD. 100%&nbsp; of normal individuals seroconverted . Among the subjects on MHD, more NDCKD (72.7%)&nbsp; seroconverted when compared to DCKD (52.3% - p value – 0.048). Non-responders had&nbsp;&nbsp; diabetes for more than 10 years.</p> <p>&nbsp;</p> <p><strong>Conclusion</strong>: among patients on MHD, those with DCKD have lower rates of seroconversion compared to those with NDCKD. Older age, less dialysis, and longer duration of diabetes adversely influenced seroconversion.</p> Nikhil Victor Dsouza, Smitha Bhat, Manjunath Kulkarni ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1080 Thu, 23 Jun 2022 19:08:21 +0000 Comparative analysis of life quality parameters of patients with a combination of stable coronary artery disease and metabolic syndrome https://rjdnmd.org/index.php/RJDNMD/article/view/1091 <p><strong>Background and aims. </strong>In recent years, the problem of comorbid course srable coronary heart disease (SCAD) and metabolic syndrome (MS) has increased significantly due to their high medical and social significance, high costs for diagnosis and treatment, reduced quality of life (QOL). The aim of the study was to conduct a comparative analysis of QOL of patients with SCAD on the background of MS in comparison to a group of patients with isolated SCAD.</p> <p><strong>Material</strong> <strong>and</strong> <strong>methods</strong><strong>. </strong>The study included 52 patients with MS in combination with SCAD and 50 patients with SCAD. QOL of all patients was assessed using the general medical questionnaire – the Medical Outcomes Study 36-Item (SF-36) of a specialized questionnaire for patients with stable angina pectoris SAQ (Seattle Angina Questionnaire).</p> <p><strong>Results.</strong> The study found a significantly lower level of QOL in patients with comorbid course of MS and SCAD in both psychological and physical components of health (by SF-36 questionnaire) compared with patients with SCAD (p&lt;0.05). According to the SAQ questionnaire, it was proved that in patients with MS in combination with SCAD the QOL was statistically significantly lower on all scales relative to the group of patients with isolated SCAD (by 8.76&nbsp;%) (p &lt;0.05).</p> Liliya S. Babinets, Nataliia A. Melnyk ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1091 Thu, 23 Jun 2022 19:15:54 +0000 The interrelation between cardiometabolic risk biomarkers and clinical features of chronic coronary syndrome in patients with non-alcoholic fatty liver disease https://rjdnmd.org/index.php/RJDNMD/article/view/1107 <p><strong>Summary</strong><strong>. </strong>Non-alcoholic fatty liver disease (NAFLD) is considered to be one of the states associated with metabolic syndrome, that significantly worsens the course of chronic coronary syndrome (CCS). Nowadays it is important to determine the common mechanisms of CCS and NAFLD progression in order to develop a comprehensive and individualized approach to the management of patients with this comorbid pathology.</p> <p><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </strong><strong>The a</strong><strong>im</strong> <strong>of</strong> <strong>the</strong> <strong>study –</strong> to study the interrelation between metabolic, pro-inflammatory disorders’ biomarkers and endothelial dysfunction with the clinical features of CCS in patients with NAFLD.</p> <p><strong>Materials and Methods</strong><strong>.</strong> The monocenter double open study in parallel groups co sex. All patients underwent biochemical study to determine the parameters of carbohydrate, lipid spectrum, functional state of the liver, enzyme-linked immunosorbent assay to determine the content of biomarkers (adiponectin, resistin, insulin, asymmetric dimethylarginine, highly sensitive C-reactive protein), daily Holter ECG monitoring and two-dimensional echocardiography.</p> <p><strong>Results and Discussion</strong>. In CCS patients with NAFLD a significant decrease in serum adiponectin level was found (by 60 % if compared to the selected control group, and by 31.6 % if compared to the comparison group; p&lt;0.05) and an increase in resistin level (by 48 % if compared to the selected control group, and by 27 % if compared to the comparison group; p&lt;0.05). In patients of the main group the ratio of adiponectin/resistin was 4.89 times lower if compared to healthy individuals and 1.48 times lower if compared to CCS patients without liver pathology (p&lt;0.05). There was a significant increase in the HOMA-IR index in CCS patients with NAFLD if compared to both healthy individuals and CCS patients without NAFLD – 5 times and 2.35 times, respectively (p&lt;0.05). Correlation analysis revealed the interrelation between adipocytokine imbalance, endothelial dysfunction markers, systemic inflammation with metabolic disorders, and indicators of liver damage in patients with CCS comorbid with NAFLD. It was found that the level of adiponectin, resistin, ADMA, adiponectin/resistin ratio, HOMA-IR index infuences the relative risk of development of atherogenic dyslipidemia, diastolic dysfunction of the LV accompanied by LV hypertrophy, vegetative imbalance, ischemic changes, and thickening of the intima-media complex development.</p> <p><strong>Conclusions</strong><strong>.</strong> In CCS patients with NAFLD, if compared to patients without NAFLD, a decrease in adiponectin, an increase in resistin, ADMA, HF-CRP, HOMA-IR index is observed. The obtained results of correlation analysis and estimation of relative risk confirm the clinical and pathogenetic role of the identified cluster of metabolic, pro-inflammatory disorders and endothelial dysfunction in the progression of CCS with&nbsp;&nbsp; concomitant NAFLD.</p> Natalia Serhiivna Mykhailovska, Iryna Olehivna Stetsiuk, Liubov Evhenivna Miniailenko, Tamila Olehivna Hawker, Oksana Oleksandrivna Lisova, Ganna Volodymyrivna Grytsay, Oksana Volodymyrivna Shershnyova, Neonila Ivanivna Korylchuk, Svitlana Stepanivna Riabokon ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1107 Thu, 23 Jun 2022 19:22:22 +0000 Study to estimate the role of blood groups in the development of cardiovascular complications among SARS Cov 2 patients – A cross-sectional study https://rjdnmd.org/index.php/RJDNMD/article/view/1113 <p><strong>Background</strong>: SARS Cov 2 virus infection has led to the development of morbidities and mortalities throughout the world in the form of a pandemic<strong>. Aim:</strong> To find out the role of blood group antigens in the development of cardiovascular complications among those infected with SARS Cov 2 infection. <strong>Materials and methods</strong>:Details of 134 patients were collected who were infected with SARS Cov 2 and admitted in Chettinad Hospital and Research Institute. <strong>Results:</strong> There was no significant role of blood groups in the severity of Covid infection or in the development of cardiovascular risks among Covid patients.</p> Santhini Gopalakrishnan, Sumathy S ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1113 Thu, 23 Jun 2022 19:25:18 +0000 Standardizing the collection and measurement of glucose in saliva and its relationship with blood glucose concentration https://rjdnmd.org/index.php/RJDNMD/article/view/1114 <p><strong><em>Title-</em></strong><strong> STANDARDIZING COLLECTION AND MEASUREMENT OF GLUCOSE IN SALIVA AND ITS RELATIONSHIP WITH BLOOD GLUCOSE CONCENTRATION.</strong></p> <p><strong><em>Mithra Nidarsh Hegde<sup>1</sup>, Shanbhag Pratheek Venkatesh<sup>2</sup>, </em></strong><strong><em>Suchetha kumari N<sup>3</sup>, </em></strong><strong><em>Prajna Bhandary<sup>4</sup></em></strong></p> <p>1-Vice Dean and Professor, department of conservative dentistry and endodontics, AB Shetty Memorial Institute of dental Sciences, NITTE deemed to be university, Mangaluru, India</p> <p>2- Post graduate, department of conservative dentistry and endodontics, AB Shetty Memorial Institute of dental Sciences, NITTE deemed to be university, Mangaluru, India</p> <p>3- Professor, department of biochemistry and principal scientific officer, central research laboratory, KS Hegde&nbsp; medical academy , NITTE deemed to be university, Mangaluru, India</p> <p>4- Research assistant, central research laboratory, KS Hegde Medical Academy, NITTE deemed to be university, Mangaluru, India</p> <p><strong><em>Abstract-</em></strong></p> <p><strong><em>Background-</em></strong> Given that diabetes has been declared a pandemic of non-communicable diseases, a chair-side, non-invasive, less technique-sensitive, cost-effective approach that reduces discomfort, anxiety and fear among the general population and encouraging them to monitor the level of glucose on a regular basis should be considered.</p> <p><strong><em>Methods-</em></strong> All salivary samples were collected 2 hours after breakfast between 9-11am. Volunteers were asked to sit upright on the dental chair and 2ml of saliva were collected in sterile fluoridated container. The standard salivary glucose level were measured using O-Toluidine reagent method using double beam spectrophotometer.</p> <p><strong><em>Results</em></strong><strong>-</strong> The study found out that the salivary glucose levels are measured more accurately using O-toluidine reagent method. 1-5ug/ml of glucose standard solution is used for standardising normal salivary glucose levels using O-Toluidine reagent method and measuring the optical density using double beam spectrophotometer.</p> <p><strong><em>Conclusions-</em></strong> The study concluded that the normal salivary glucose level were measured more accurately by O-toluidine reagent method using double beam spectrophotometer. Therefore, it can be concluded that the standard glucose solution needed for measuring normal glucose level in the blood and saliva is in the ratio of 1:200 -1:160.</p> <p><strong><em>Keywords</em></strong>– Standard glucose solution, Saliva, Salivary glucose level, O-Toluidine reagent, Double beam spectrophotometer.</p> Pratheek Venkatesh Shanbhag, Mithra N. Hegde, Suchetha Kumari N, Prajna Bhandary ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1114 Thu, 23 Jun 2022 19:48:03 +0000 Nrf2 induction alleviates metabolic disorder and systemic inflammatory response in rats under a round-the-clock lighting and high-carbohydrate–lipid diet https://rjdnmd.org/index.php/RJDNMD/article/view/1122 <p><strong><em>Background and aims</em></strong><strong>:</strong> The aim of this work is to study the effect of Nrf2 inductor dimethyl fumarate (DMF) on metabolic disorder and systemic inflammatory response in rats exposed to round-the-clock lighting (RCL) and kept on the high-calorie carbohydrate-lipid diet (HCLD). <strong><em>Materials and methods</em>:</strong> The experiment was performed on 21 Wistar white male rats. Control rats (Group 1) were fed standard control chow and kept on 12/12 hours light / dark cycle. Rats of the Group 2 were kept on a HCLD for 8 weeks and exposed to RCL, thus simulating metabolic syndrome development. The animals of the Group 3 received DMF in 10% dimethyl sulfoxide solution in a dose of 15 mg/kg intraperitoneally 3 times a week starting on the 30<sup>th</sup> day of the experiment. <strong><em>Results:</em></strong> DMF administered against the RCL and HCLD background lowered the serum glucose, insulin, cholesterol of very low density lipoproteins and triglycerides levels and elevated the cholesterol of high-density lipoproteins concentration compared with the findings of Group 2. Administering DMF under RCL and HCLD reduced serum tumour necrosis factor-alpha, С-reactive protein, and blood tiobarbituric acid-reacting compounds concentration compared to the results in Group 2. <strong><em>Conclusions</em>:</strong> The induction of Nrf2 signalling system can be regarded as a potential target in the experimental therapy of metabolic syndrome, triggered by the disturbance of light / dark cycle and Western diet.</p> Yurii D. Frenkel, Valerii S. Cherno, Vitalii Kostenko ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1122 Thu, 23 Jun 2022 19:53:36 +0000 A cross-sectional study to estimate the prevalence of metabolic and pre-metabolic syndrome among young adults in rural population of Chengalpattu district https://rjdnmd.org/index.php/RJDNMD/article/view/1120 <p><strong>Abstract</strong></p> <p><strong>Background</strong></p> <p>Metabolic&nbsp;syndrome&nbsp;is&nbsp;a&nbsp;globally&nbsp;trending&nbsp;health&nbsp;care&nbsp;problem&nbsp;with&nbsp;increased&nbsp;risk&nbsp;of&nbsp;developing&nbsp;cardiovascular&nbsp;disease,&nbsp;insulin&nbsp;resistance,&nbsp;stroke&nbsp;etc.The&nbsp;prevalence&nbsp;of&nbsp;this&nbsp;syndrome&nbsp;is&nbsp;soaring&nbsp;among&nbsp;Indians,&nbsp;especially&nbsp;in&nbsp;the&nbsp;rural&nbsp;background&nbsp;area.&nbsp;However,&nbsp;not&nbsp;many&nbsp;studies&nbsp;were&nbsp;focused&nbsp;on&nbsp;young&nbsp;adults&nbsp;in&nbsp;the&nbsp;rural&nbsp;areas&nbsp;of&nbsp;Tamilnadu.&nbsp;Hence,&nbsp;the&nbsp;present&nbsp;study&nbsp;was&nbsp;done&nbsp;to&nbsp;estimate&nbsp;the&nbsp;prevalence&nbsp;of&nbsp;Metabolic&nbsp;Syndrome&nbsp;and&nbsp;Premetabolic&nbsp;syndrome&nbsp;between&nbsp;20-40&nbsp;years&nbsp;and&nbsp;to&nbsp;assess&nbsp;the&nbsp;association&nbsp;between&nbsp;the&nbsp;life&nbsp;style&nbsp;risk&nbsp;factors&nbsp;and&nbsp;metabolic&nbsp;syndrome&nbsp;in&nbsp;rural&nbsp;population&nbsp;of&nbsp;Chengalpattu&nbsp;district.</p> <p><strong>Material&nbsp;and&nbsp;methods</strong></p> <p>It&nbsp;was&nbsp;an&nbsp;age&nbsp;and&nbsp;sex&nbsp;matched,&nbsp;cross-sectional&nbsp;study&nbsp;done&nbsp;at&nbsp;Chettinad&nbsp;hospital&nbsp;and&nbsp;research&nbsp;institute,&nbsp;Kelambakkam.&nbsp;A&nbsp;total&nbsp;of&nbsp;360&nbsp;apparently&nbsp;healthy&nbsp;subjects,&nbsp;who&nbsp;has&nbsp;come&nbsp;for&nbsp;Master&nbsp;health&nbsp;check&nbsp;up&nbsp;were&nbsp;included&nbsp;in&nbsp;the&nbsp;study.&nbsp;Fasting&nbsp;blood&nbsp;sample&nbsp;was&nbsp;collected&nbsp;and&nbsp;measured&nbsp;for&nbsp;Plasma&nbsp;glucose,&nbsp;serum&nbsp;triglycerides,&nbsp;and&nbsp;high&nbsp;density&nbsp;lipoprotein.&nbsp;The&nbsp;study&nbsp;participants&nbsp;were&nbsp;then&nbsp;categorized&nbsp;based&nbsp;on&nbsp;NCEP&nbsp;ATP&nbsp;III&nbsp;guidelines.&nbsp;Data&nbsp;were&nbsp;presented&nbsp;as&nbsp;percentage(%).&nbsp;Student&nbsp;independent&nbsp;t&nbsp;test&nbsp;was&nbsp;used&nbsp;to&nbsp;compare&nbsp;groups&nbsp;with&nbsp;continuous&nbsp;variables&nbsp;and&nbsp;for&nbsp;categorical&nbsp;variables,&nbsp;chi-square&nbsp;test&nbsp;was&nbsp;used.&nbsp;All&nbsp;Statistical&nbsp;analysis&nbsp;was&nbsp;carried&nbsp;out&nbsp;using&nbsp;SPSS&nbsp;software.</p> <p><strong>Results</strong></p> <p>The&nbsp;prevalence&nbsp;of&nbsp;Metabolic&nbsp;syndrome&nbsp;was&nbsp;36.6%&nbsp;of&nbsp;which&nbsp;males&nbsp;presents&nbsp;more&nbsp;with&nbsp;37.8%&nbsp;and&nbsp;females&nbsp;35.4%.&nbsp;The&nbsp;prevalence&nbsp;of&nbsp;Premetabolic&nbsp;syndrome&nbsp;was&nbsp;27.3%&nbsp;among&nbsp;which&nbsp;25.2%&nbsp;were&nbsp;males&nbsp;and&nbsp;29.3%&nbsp;were&nbsp;females.&nbsp;Odds&nbsp;risk&nbsp;of&nbsp;MS&nbsp;was&nbsp;significantly&nbsp;higher&nbsp;among&nbsp;males&nbsp;with&nbsp;increased&nbsp;waist&nbsp;circumference,&nbsp;alcohol&nbsp;drinking,&nbsp;and&nbsp;smoking&nbsp;habit.&nbsp;The&nbsp;Odds&nbsp;risk&nbsp;of&nbsp;MS&nbsp;among&nbsp;females&nbsp;was&nbsp;significantly&nbsp;higher&nbsp;with&nbsp;those&nbsp;of&nbsp;alcohol&nbsp;and&nbsp;smoking&nbsp;usage,&nbsp;family&nbsp;history&nbsp;of&nbsp;hypertension,&nbsp;high&nbsp;fasting&nbsp;triglycerides,&nbsp;and&nbsp;low&nbsp;HDL&nbsp;cholesterol&nbsp;in&nbsp;the&nbsp;serum.</p> <p><strong>Conclusion</strong></p> <p>The&nbsp;huge&nbsp;surge&nbsp;in&nbsp;the&nbsp;prevalence&nbsp;rate&nbsp;of&nbsp;Metabolic&nbsp;syndrome&nbsp;and&nbsp;Premetabolic&nbsp;syndrome&nbsp;has&nbsp;to&nbsp;be&nbsp;intervened&nbsp;by&nbsp;implementing&nbsp;various&nbsp;health&nbsp;care&nbsp;policies&nbsp;so&nbsp;that&nbsp;the&nbsp;mortality&nbsp;and&nbsp;morbidity&nbsp;can&nbsp;be&nbsp;significantly&nbsp;reduced&nbsp;among&nbsp;the&nbsp;young&nbsp;adults&nbsp;of&nbsp;the&nbsp;rural&nbsp;population.</p> <p><strong>Key&nbsp;words</strong></p> <p>Metabolic&nbsp;syndrome,&nbsp;Premetabolic&nbsp;syndrome,&nbsp;rural&nbsp;population</p> Sumathy Purandar, Anuradha Ganesan ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1120 Thu, 23 Jun 2022 19:59:42 +0000 Histological analysis of the placenta in patients with preeclampsia, taking into account the proposed treatment https://rjdnmd.org/index.php/RJDNMD/article/view/1123 <p><strong>Background and aims:</strong> Preeclampsia is a critical condition syndrome that occurs during pregnancy and may lead to the complications and fetal lesions. The aim of this study is to optimize the algorithm for the treatment of patients at high risk preeclampsia associated with metabolic syndrome by morphologically proven effects on the placental complex. <strong>Material and method:</strong> Material for histological examination was collected from the peripheral, medial and central parts of the placental disc and placental amnion. Tissue fragments were processed in a LogosOne histoprocessor and embedded in paraffin blocks.<strong> Results:</strong> In the first study group (standard protocol treatment) there were histological changes which should be regarded as morphological markers of functional disorders in the "mother-placenta-fetus" system that accompany late preeclampsia. Metaplasia of the cylindrical epithelium of the fetal surface into a multilayered flat, increased proliferative activity of epitheliocytes whith formation of plaque-like structures and growths in the placental amnion was observed. In the second study group (modified treatment) the intensity and prevalence of pathological changes were significantly lower than in the first study group. The citral structure of the amniotic epithelium was preserved, desquamation was not observed. <strong>Conclusions:</strong> Based on the results of histological examination, it can be stated that complex modified therapy of preeclampsia associated with metabolic syndrome allows to normalize the structure of the placenta, which is objectively confirmed by reducing the frequency of alternative changes in the placental amnion, epithelium and stroma of chorionic villi.</p> <p>Keywords: preeclampsia, placenta, histology, metabolic syndrome, treatment.</p> Ulyana Franchuk, S. Khmil, Yu. Orel, M. Franchuk, L. Malanchuk ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1123 Thu, 23 Jun 2022 20:03:31 +0000 Metabolic syndrome: Correlation between main hormones and oxidative stress parameters https://rjdnmd.org/index.php/RJDNMD/article/view/1134 <p>Abstract <br>Background and aims: Pathogenetic aspects of the metabolic syndrome are still the subject of debate due to significant differences in the results of many studies. The aim of the study was to examine the correlation between the levels of ghrelin, leptin, insulin, adiponectin, resistin, metabolic syndrome and oxidative stress parameters in patients with obesity of varying degrees. Material and methods: It was examined 44 patients (59.10 % of women and 40.90 % of men) aged 31 to 79 years with a body mass index ˃30 kg / m2 (main group) and 12 patients (66.67% - women and 33.34% - men) aged 25 to 67 years, with a body mass index ˂25 kg / m2, which made up the control group. Plasma levels of glycated hemoglobin, glycemia, as well as total cholesterol, low and high density lipoproteins, triglycerides, eptin, ghrelin general, adiponectin, oxidized low-density lipoprotein cholesterol, resistin were measured. Results: The analysis confirmed the presence of an imbalance of orexigenic and anorexigenic hormones in obese patients, with a progressive increase in leptin, insulin and decreased levels of ghrelin, adiponectin with increasing obesity, as evidenced by the corresponding correlations. Metabolic syndrome is characterized by excessive accumulation of fat in adipose tissue, which leads to adipocyte hypertrophy, hypoxia, and the development of systemic oxidative stress. Because adipose tissue is responsible for the production of various vasoactive adipokines to modulate vascular function, adipose tissue dysfunction caused by obesity significantly contributes to the pathogenesis of cardiovascular disease. Correction of metabolic parameters and oxidative stress is a potential strategy for the prevention and treatment of cardiovascular disease.</p> Andrii Prodan, Dzhyvak V. G. ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1134 Thu, 23 Jun 2022 20:05:50 +0000 Cytomorphometric evaluation of exfoliated cells of the tongue and it’s correlation with clinical findings in type II diabetic patients https://rjdnmd.org/index.php/RJDNMD/article/view/1148 <p class="Default" style="text-align: justify; line-height: 150%;"><strong><span lang="EN-US" style="font-family: 'Times New Roman',serif;">Background and Aims: </span></strong><span lang="EN-US" style="font-family: 'Times New Roman',serif;">Exfoliative cytology is a non-invasive diagnostic method that has been used for decades to analyse cellular and nuclear alterations. Diabetes Mellitus(DM) is a common metabolic illness that results in chronic hyperglycemia, as well as significant cellular alterations in the oral mucosa, particularly in the tongue which is often neglected. Hence we carried out a quantitative and qualitative analysis of the exfoliated epithelial cells of the tongue and clinical changes that manifested in tongue associated with Uncontrolled and Controlled Type 2 Diabetes Mellitus (T2DM). <strong>Material and Methods: </strong>Tongue smears were obtained 125 in Uncontrolled T2DM (Group I), 125 Controlled T2DM (Group II) and 30 control individuals (Group III) along with clinical examination of the tongue. PAP stained smears were subjected for the analysis of nuclear area (NA), cytoplasmic area (CA) and cytoplasmic area to nuclear area ratio (C/N) using digital analyzer system and PAS stained smears were subjected for the assessment of Candida organisms. <strong>Results: </strong>Cytomorphometric assessment of the tongue smears showed increased nuclear area, decreased cytoplasmic area and decreased mean cytoplasmic area to nuclear area ratio among study group and control group, PAS stained smears showed positivity for candida organisms in 53.6% in Group I and 44.0% in Group II. Clinical correlation with cytomorphometric changes among Group I and II did not show any statistical significance in the present study. <strong>Conclusion</strong>: The results obtained from our study emphasizes the role of exfoliative cytology as a diagnostic tool in contributing to the general understanding of the changes in the tongue in patients with Uncontrolled and Controlled T2DM.</span></p> Balamanikandan P, Pushparaja Shetty, Urvashi A Shetty ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1148 Thu, 23 Jun 2022 20:07:22 +0000 Laboratory criteria for comorbid course of type 2 diabetes mellitus and thyroid dysfunction https://rjdnmd.org/index.php/RJDNMD/article/view/1149 <p><strong>Background and aims:</strong> Diabetes mellitus (DM) rates have reached endemic levels, which is a major challenge for the modern health care system; the vast majority are patients with type 2 diabetes mellitus (T2DM). The presence of other comorbidities drastically affects diabetic patient outcomes, treatment and management options. The aim of our study was to analyze routine laboratory data of type 2 diabetic patients with thyroid dysfunction and search for possible markers of comorbid course of T2DM, hypothyroidism (HT) and diffuse nontoxic goiter (DNG). <strong>Material and method:</strong> We analyzed 596 medical records of T2DM patients without thyroid dysfunction and with comorbid HT and/or DNG. <strong>Results and conclusions:</strong> Type 2 diabetic patients with comorbid HT or DNG have significantly higher BMI, increased level of HbA1c and increased ESR compared with patients with T2DM without thyroid dysfunction. The progression of lipid metabolism disorders in patients with comorbid course of t T2DM and HT is characterized by a significant decrease of HDL-C level, as well as an increase of RC and TG levels compared with T2DM only, as well as the comorbidity of type 2 diabetes mellitus and diffuse nontoxic goiter.</p> Volodymyr Musiienko ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1149 Thu, 23 Jun 2022 20:11:03 +0000 Providing preclinical prediction of diabetic retinopathy via oxidative stress levels https://rjdnmd.org/index.php/RJDNMD/article/view/1152 <p><strong>Background and aims:</strong> Although diabetic retinopathy(DR) is a disease that develops due to diabetes mellitus(DM), it also brings problems in the clinic. Depending on the diagnosis and related developments, the role of metabolites that cause stress in the cell and disorders in metabolism should be constantly reviewed. We aim to examine inflammation, oxidative stress levels, and DNA damage in DM and DR patients. <strong>Material and method:</strong> A total of 3 groups of 30 patients with a 5-year history of DM, 30 patients with a 5-year history of DR, and healthy individuals with the same demographic characteristics as both patient groups, between the ages of 45 and 65 who came to the Bezmialem Vakif University Faculty of Medicine Ophthalmology Outpatient Clinic were included. Total antioxidant status(TAS), total oxidant status(TOS), total thiol(TT), and native thiol(NT) of patients and healthy individuals by photometric methods; inflammation biomarkers interleukin-1 beta(IL-1β), interleukin-6(IL-6), and tumor necrosis factor-alpha(TNF-α) were measured by photometric methods with commercially purchased ELISA kits. <strong>Results:</strong> Oxidative stress and inflammation were statistically increased in both DR and DM patients, and antioxidant levels were statistically decreased in both DR and DM patients(p&lt;0,01). IL-1β levels(p&lt;0.001), TNF-α, and IL-6 levels(p&lt;0.01) were found statistically significant and high. DNA damage was found higher in patients with DR compared to both the control group(p&lt;0.001) and the patient group with DM(p&lt;0.001). <strong>Conclusions:</strong> Increased oxidative stress and prominent inflammation in DR patients may provide clinical guidance on the pathogenesis, prognosis, and treatment strategies of the disease.</p> Hakan Beyaztaş, Cansu Ekinci Aslanoglu, Abdulkerim Yildiz ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1152 Thu, 23 Jun 2022 20:16:29 +0000 Characteristics of certain genetical and biological properties of carcinogenesis in the development of inflammatory breast cancer with type 2 diabetes mellitus and tumor relapse https://rjdnmd.org/index.php/RJDNMD/article/view/1155 <p><strong>Introduction. </strong>&nbsp;The aim of the work the ability to increase the effectiveness of comprehensive treatment of patients with inflammatory breast cancer with type 2 diabetes mellitus by individualization based on the determination of carcinogenesis indicators of the malignant process.</p> <p><strong>The aim of the study</strong> the ability to increase the effectiveness of comprehensive treatment of patients with inflammatory breast cancer with type 2 diabetes mellitus by individualization based on the determination of carcinogenesis indicators of the malignant process.</p> <p><strong>Materials and research methods</strong>. We examined 80 patients with Inflammatory breast cancer with type 2 diabetes mellitus, who were determined MSI at the loci of BAT 25; BAT 26, Ki-67 proliferation index, and p53 gene mutation and plasma glucose level control.&nbsp; To assess the effectiveness of treatment depending on the biological properties of the tumor, in tumor tissue studied the expression levels of p53 protein, the index of proliferative activity of Ki-67, determined the presence of MSI at the loci BAT-25 and BAT-26 and their interconnection and dependence on each other.</p> <p><strong>Results. </strong></p> <p>It is established in this case; this is realized due to the inability of the mutant form of the p53 gene to synthesize the desired Quaternary configuration of the corresponding protein with the subsequent inability of the latter to stop the process of DNA replication. It was found that the proliferative activity of tumor cells in inflammatory breast cancer had a clear relationship with MSI at both studied chromosome loci. The presence of MSI is somehow associated with hyperglycosemia and is an aggravating factor in the course of inflammatory breast cancer with a poorer response to specific therapy.</p> Michael Ivanovich Sheremet, Bagmut Irina Yuriivna, Movchan Oleksii Volodimirovich, Smolanka Ivan Ivanovich, Lyashenko Andriy Oleksandrovich, Dosenko Irina Viktorivna, Loboda Anton Dmitrovich, Kolisnyk Igor Leonidovich ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1155 Thu, 23 Jun 2022 20:29:53 +0000 Dyslipidemia as a predictor of essential arterial hypertension depending on AGTR1 (RS5186) and VDR (RS2228570) genes polymorphism https://rjdnmd.org/index.php/RJDNMD/article/view/1156 <p><strong>Background and aims:</strong> The purpose of the work was to study lipid disorders and essential arterial hypertension (EAH) risk depending on <em>AGTR1 (rs5186)</em>, <em>VDR (rs2228570)</em> genes polymorphism. <strong>Material and method:</strong> 100 subjects with EAH and target-organ damaging (2<sup>nd</sup> stage), moderate, high or very high cardiovascular risk were involved in the case-control study. Control group consisted of 60 healthy individuals of relevant gender and age. Lipid panel was determined by colorimetric enzymatic&nbsp;method. Gene polymorphism of AGTR1 (rs5186) and VDR (rs2228570) was detected by polymerase chain reaction. <strong>Results:</strong> The EAH risk increases in C-allele carriers of AGTR1 (rs5186) gene at hypercholesterolemia 1.5 times, at an increase in low-density lipoprotein cholesterol (LDL-C) and atherogenic index (AI) – 1.58 and 2.12 times, respectively. Risk of EAH increases at hypertriglyceridemia 1.89 times and at increase in LDL-C – 1.26 times in AA-genotype carriers of VDR (rs2228570) gene. Risk of EAH synergistically escalates almost twice in A-allele carriers of VDR (rs2228570) gene with a decrease of high-density lipoprotein cholesterol (HDL-C) and increase of AI, significantly in AG-carriers, though. <strong>Conclusions:</strong> The EAH risk escalates at hypercholesterolemia, elevated LDL-C and AI in C-allele carriers of AGTR1 (rs5186) gene, likewise at decreased HDL-C, increased AI in A-allele carriers of VDR (rs2228570) gene in the observed.</p> Marianna Marianna ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1156 Thu, 23 Jun 2022 20:31:24 +0000 Serum levels of adiponutrin in differentiating non-alcoholic steatohepatitis from simple steatosis in non-alcoholic fatty liver disease patients https://rjdnmd.org/index.php/RJDNMD/article/view/1169 <p><strong>Background and aims: </strong>Nonalcoholic Fatty liver disease is one of the chronic liver disease, characterized by an unusual accumulation of fats in hepatocytes. It’s a multifactorial disease and the pathogenesis is not completely known. The genetic association is seen, where the Patatin-like phospholipase domain-containing protein 3(PNPLA3) is the most relevant genetic association. PNPLA3 encodes for a protein called adiponutrin (ADPN), which has triglyceride hydrolase and acyltransferase activity. Hepatic fat accumulation results due to excess lipogenesis or decreased fat export. Liver biopsy is the only gold standard available for differentiating the stages of NAFLD, but it’s an invasive procedure that may lead to potential infections. The study aimed to evaluate serum levels of ADPN in stages of NAFLD.</p> <p><strong>Material and methods:</strong> This is an observational study comprised of 60 NAFLD cases, among which 10 subjects were stage 1 (Simple steatosis) and 50 subjects stage 2 (Nonalcoholic steatohepatitis-NASH). Blood samples were collected from the subjects and serum ADPN levels were assessed by enzyme-linked immunosorbent assay.</p> <p><strong>Results:</strong> The serum levels of ADPN were significantly decreased in NASH as compared to simple steatosis (<em>p</em>&nbsp;&lt; 0.001).</p> <p><strong>Conclusions:</strong> ADPN serum level was inversely associated with the progression of NAFLD.</p> <p>&nbsp;</p> Mayuri Shukla, Mamatha Kunder, Prabhakar Kamarthy, Sharath Balakrishna ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1169 Thu, 23 Jun 2022 20:34:27 +0000 Taste disorders in diabetes – An insight https://rjdnmd.org/index.php/RJDNMD/article/view/1037 <p>Taste sensation or gustation is an important feature that determines the food selection and nutrition of human beings. Gustation is a complex process that takes place in the taste buds resulting in the chemosensory perception of the food substances. Humans have the ability to perceive a variety of tastes such as sweet, sour, bitter, salty and umami. Taste disorder is a broad term used to denote any alteration in the perception of tastes. Taste disorder is one of the commonly encountered oral manifestation of both type 1 and type 2 diabetes mellitus, which is often ignored. These disorders can have a direct impact on the glycemic control of the diabetic patients. There are various chairside investigations that can help in the detection of taste disorders. Early detection and appropriate management of taste disorders in patients with diabetes is essential for a better control of the glycemic levels.</p> Mohamed Faizal Asan, G Subhas Babu, Vidya Ajila, Sonika Achalli ##submission.copyrightStatement## https://rjdnmd.org/index.php/RJDNMD/article/view/1037 Thu, 23 Jun 2022 20:36:10 +0000