Long non-coding RNA-H19 and miRNA-29a expression in type 2 diabetes mellitus patients

Mohammed Abdulfatah Al-ayash; Mohammed Ibrahim Nader

Mohammed Abdulfatah Al-ayash Department of Medical Laboratories, Al-Furat General Hospital, Ministry of Health, Baghdad, Iraq
Mohammed Ibrahim Nader Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, University of Baghdad, Baghdad, Iraq

Keywords: lncRNA H19, miRNA29-a, q PCR, T2DM

Abstract

Diabetes mellitus type 2 is one of the most prevalent illnesses. This study aims to shed light on the expression levels of the long non-coding RNA H19 and miRNA-29a, which are important in the diagnosis and pathogenesis of T2DM. The study included the collection of samples from 100 patients with type 2 diabetes attending AL-Furat General Hospital in Baghdad, following medical investigation, and 100 apparently healthy individuals as a control group, from July 2023 to November 2023. The Relative H19 expression levels were increased in the patient’s group (fold 1.38) vs. controls (fold 1.00) with a highly significant difference (p≤0.01), similar to miRNA29-a levels, which were also increased in the patient’s group (fold 2.17) with a highly significant difference (p<0.01). The analysis of H19 revealed an area under the curve of 0.86 (p<0.001), with 68% sensitivity and 96% specificity. The positive predictive value was 84.6%. The results of the analysis of miRNA-29a showed that the area under the curve was 0.91 and p<0.001, with 86% sensitivity and 92% specificity. The positive predictive value was 98%. The severity of diabetes 2 patients is linked to high lncRNA H19 expression and increased miRNA-29a expression, suggesting a potential role for miRNA-29a in the disease’s etiology.