SIRT1 LEVEL DIFFERENCES AMONG OLD RATS, STREPTOZOTOCIN-INDUCED DIABETIC RATS, AND D-GALACTOSE-INDUCED AGING RATS

Keywords: aging, rat model, streptozotocin, d-galactose, SIRT1

Abstract

Introduction: The Sirtuin 1 (SIRT1) level is suspected to be lower in older ages. In a study of rats, aging can be induced by D-galactose. Various degenerative diseases can also develop from streptozotocin induction (rat models for diabetes mellitus). This study aimed to verify SIRT1 level differences among old rats, D-galactose-induced diabetic rats and streptozotocin- induced aging rats. Material and Methods: A total of 20 female Wistar rats aged four months was divided into five groups of 4 rats each. Group K1 was directly terminated, K2 and K3 were kept until 18 and 33 months old, respectively, and later terminated. Rats in the K4 group were induced with streptozotocin at a dose of 65 mg/kg BW and nicotinamide at 120 mg/ kg BW and kept for one month. Rats in the K5 group were induced by 300 mg/kg BW of D-galactose for one month. Blood specimens were taken to examine SIRT1 levels using ELISA. The statistical test used in this study was the Kruskal-Wallis test (95% confidence level). Results: SIRT1 levels in rats aged 4, 18, and 33 months, diabetic rat models, and D-galactose-induced aging were 0.43 ± 0.12, 0.58 ± 0.15, 0.30 ± 0.01, 0.25 ± 0.13, and 0.21 ± 0.05, respectively (p=0.028). Conclusions: D-galactose- induced aging rat models had the lowest SIRT1 level compared to 33-month-old rats and diabetic rat models.

Published
2020-03-15
How to Cite
Fidianingsih, Ika, and Dwi Ahsani. 2020. “SIRT1 LEVEL DIFFERENCES AMONG OLD RATS, STREPTOZOTOCIN-INDUCED DIABETIC RATS, AND D-GALACTOSE-INDUCED AGING RATS”. Romanian Journal of Diabetes Nutrition and Metabolic Diseases 27 (1), 39-44. http://rjdnmd.org/index.php/RJDNMD/article/view/725.
Section
Original Research Articles