The effect of Purslane and Aquilaria malaccensis on insulin-resistance and lipid peroxidation in High-fructose diet Rats

Abstract

The aim of the present study was to evaluate the effect of purslane (P. oleracea)  and Aquilaria malaccensis (A. malaccensis) methanol extracts against High-fructose and high-fat diet induced insulin-resistance, lipid peroxidation and tissues dysfunction in Rats. Females albino Wistar rats were divided into 6 groups (n=5) as control, Insulin resistance rats (IR), IR+Po, IR+Am, IR+Po+Am and IR+Met groups. Insulin resistance (IR) in rats was induced by diet with either a high fructose (60% fructose and a high fat diet (60% kcal fat) for 70 days. P. oleracea (Po) and A. malaccensis (Am) methanol extracts and metformin drug  were supplemented orally (400 mg/kg bw, 200 mg/kg bw and 300 mg/kg bw) respectively for four weeks. Methanol extracts of plants were prepared and phytochemicals were analyzed by standard methods. Blood glucose level, lipids profile, lipid peroxidation and some biochemical parameters were assessed.  Obtained results revealed that IR induction caused a significant increase in blood glucose, plasma and tissues lipids profile, urea and creatinine concentrations, GOT, GPT, and ALP activities compared to the control group. While total protein concentration was significantly lower. Additionally, a significant increase of MDA level in IR group than those of the control. Methanol extracts of P. oleracea  and A. malaccensis treatment ensured a partial correction of the previous parameters. The results of the present investigation indicated that P. oleracea  and A. malaccensis possesses the ability to control the lipid peroxidation and biochemical disruption associated with insulin resistance.