Mitochondrial dysfunction of mesenchymal stem cells isolated from blood with type 2 diabetic patients

Svetlana Mykolaivna Gramatiuk; Irіna Yuriivna Bagmut; Michael Ivanivich Sheremet; Julia Viktorivna Ivanova; Igor Leonidovich Kolisnik; Maria Sergiivna Gramatiuk; Dragos Cretoiu; Vitaliy Vasilyevich Maksymyuk; Volodimir Volodimirovich Tarabanchuk; Oleksandr Volodimirovich Lazaruk; Petro Vasilyevich Moroz

Svetlana Mykolaivna Gramatiuk Institute of Cellular Biorehabilitation, Ukraine Association of Biobank, Kharkiv 61000, Pushkinska str. 44, Ukraine
Irіna Yuriivna Bagmut Kharkiv Medical Academy of Postgraduate Education, Kharkiv, Ukraine
Michael Ivanivich Sheremet Surgery Department No1, Bukovinian State Medical University, Ukraine
Julia Viktorivna Ivanova State Institution «Zaytsev V.T. Institute of General and Urgent Surgery of National Academy of Medical Sciences of Ukraine», Kharkiv, Balakirev str. 1, Ukraine
Igor Leonidovich Kolisnik Kharkiv Medical Academy of Postgraduate Education, Kharkiv, Ukraine
Maria Sergiivna Gramatiuk Institute of Cellular Biorehabilitation, Ukraine Association of Biobank, Kharkiv 61000, Pushkinska str. 44, Ukraine
Dragos Cretoiu MD, Phd, Dr. MD – Centrul de excelenta M.F. al I.N.S.M.C., Bucuresti, Romania
Vitaliy Vasilyevich Maksymyuk Surgery Department No1, Bukovinian State Medical University, Ukraine
Volodimir Volodimirovich Tarabanchuk Surgery Department No1, Bukovinian State Medical University, Ukraine
Oleksandr Volodimirovich Lazaruk Department of Pathology (Pathology and Forensic Medicine), Bukovinian State Medical University, Ukraine
Petro Vasilyevich Moroz Surgery Department No1, Bukovinian State Medical University, Ukraine

Keywords: Noninvasive prenatal testing, cell-free fetal DNA, magnetic-activated cell sorting, gestational diabetes.

Abstract

The aim of the study is to focus on mitochondrial dysfunction, in the context of NASH, mitochondrial function in stem cells is likely to be impaired. Materials and methods Mesenchymal stem cell separated of peripheral blood from diabetes 2 type (DT2) patients was collected in the context of a clinical protocol authorized by the local Ethics Committee of Ukraine Association of Biobank (Ukraine), with a license from the Ministry of Health of Ukraine 04/10/2018 №1813 and 27/03/2019 №1231 by the national competent authority for biobank cord blood, cell and, tissue therapy. The study population (n = 96) was represented by diabetic patients from SI «ZIGUS NAMSU» in Kharkiv, Ukraine, and healthy volunteers. Patients were divided into two groups: group I
consisted of patients with diabetes 2 type (DT2), group II - patients with DT2 complicated course of NASH (DT2 + NASH). The control group consisted of 25 conditionally healthy persons (men and women) of the same age. Conclusion: In the modern scientific space, various directions have been proposed in the diagnosis of metabolic syndrome and the treatment of D2T.