Characteristics of cytokine status in intra-abdominal infection with underlying diabetes mellitus: An experimental study

  • Fedir Vasilyevich Grynchuk Department of Surgery №1, Bukovinian State Medical University, Chernivtsi, Ukraine
  • Ihor Yulievich Polianskiy Department of Surgery №1, Bukovinian State Medical University, Chernivtsi, Ukraine
  • Andriy Fedirovich Grynchuk Department of Surgery №1, Bukovinian State Medical University, Chernivtsi, Ukraine
  • Michael Ivanovich Sheremet Surgery department of Bucovinian medical University
  • Vitaliy Vasilyevich Maksymyuk
  • Volodymyr Volodymyrovich Tarabanchuk Department of Surgery №1, Bukovinian State Medical University, Chernivtsi, Ukraine
  • Valeriy Stepanovich Vasylovych Department of Surgery, City polyclinic № 2, Chernivtsi, Ukraine
Keywords: diabetes mellitus, intraabdominal infections, cytokines.

Abstract

Background and aims: Diabetes mellitus (DM) is known to have an increased susceptibility to infections and poor treatment outcomes, intraabdominal infections (IAI), in particular. Changes in the functioning of mechanisms for regulating inflammation under the DM influence are the main reason. It is crucial to study the state of the cytokine network. Since cytokines (Ctk) play a major role in the immune response and inflammation regulation. Our study aims to compare changes in cytokine status in IAI with underlying DM in the experiment. Material and method: The object of the studies is 100 mature albino nonlinear rats. Their weight is 180–200 g. To create the IAI model, animals have undergone a transesophageal gastric perforation. DM has been induced by subcutaneous administration of a 1.6% alloxan solution in distilled water at a dose of 16 mg per 100 g of body weight. The animals have been divided into the following groups: intact animals, animals with DM models, intact animals with peritonitis models (group 1), animals with peritonitis models with underlying DM (group 2). IAI has been modeled 3 months following the DM simulation. The blood has been taken from the cervical vein both before the IAI simulation and in 6, 12, 24, 48 hours of its simulation. The content of tumor necrosis factor α (TNFa) and interleukins (IL) 2, 6, and 10 in blood plasma have been studied. Results: The content of proinflammatory Ctk in animals with DM models has been significantly higher than in intact ones. The IL 10 content has been lower. After 6 hours of IAI modeling, the plasma content of all Ctk, most notably TNFa, has significantly
increased in group 1. The amount of TNFa has slightly changed in group 2. The amount of IL 2, IL 6 (table 4) has significantly increased. The amount of IL 10 has increased more than 50 times. The amount of TNFa, IL 6, and 10 have been significantly decreased. The amount of IL has remained at the same level. The number of all Ctk has immediately decreased in group 2. The TNFa number has been the lowest. After 24 hours, the number of TNFa has slightly increased in group 1 since the IAI simulation. The number of other Ctk is unlikely to have been decreased. IL 2 is the most pronounced to have been decreased. The number of TNFa has remained quite the same in group 2. The number of remaining Ctk has continued to decrease rapidly. After 48 hours, the number of TNFa and IL 10 have decreased in group 1 since the IAI simulation. Moreover, the number of IL 10 has become less than the initial indicators. The number of IL 6 has slightly changed, and the number of IL 2 has increased significantly. The number of IL 6 has increased significantly in group 2. The number of IL 6 has increased slightly, while the number of TNFa and IL 10 has remained almost unchanged. Conclusions: DM modeling causes a disparity in cytokine status. The reason is a higher number of pro-inflammatory Ctk in the background of a less number of IL 10. Intact animals with IAI models have shown signs of cyclical changes in the activity of various mechanisms of inflammatory regulation within 48 hours. At the same time, in 48 hours, there have been signs of impaired function of the regulation mechanisms of the immune response. There have been differences in the functioning of various mechanisms of inflammatory regulation in 6 hours in animals with IAI models with underlying DM. There have been signs of immunosuppression in 12 hours. These changes have been progressing later on and the signs of immune insufficiency have been shown in 48 hours.

Published
2021-10-31
How to Cite
Grynchuk, Fedir Vasilyevich, Ihor Yulievich Polianskiy, Andriy Fedirovich Grynchuk, Michael Sheremet, Vitaliy Vasilyevich Maksymyuk, Volodymyr Volodymyrovich Tarabanchuk, and Valeriy Stepanovich Vasylovych. 2021. “Characteristics of Cytokine Status in Intra-Abdominal Infection With Underlying Diabetes Mellitus: An Experimental Study”. Romanian Journal of Diabetes Nutrition and Metabolic Diseases 28 (3), 255-60. https://rjdnmd.org/index.php/RJDNMD/article/view/1054.