Standardizing the collection and measurement of glucose in saliva and its relationship with blood glucose concentration

  • Pratheek Venkatesh Shanbhag Postgraduate
  • Mithra N. Hegde
  • Suchetha Kumari N
  • Prajna Bhandary
Keywords: Standard glucose solution, Saliva, Salivary glucose level, O-Toluidine reagent, Double beam spectrophotometer.

Abstract

Title- STANDARDIZING COLLECTION AND MEASUREMENT OF GLUCOSE IN SALIVA AND ITS RELATIONSHIP WITH BLOOD GLUCOSE CONCENTRATION.

Mithra Nidarsh Hegde1, Shanbhag Pratheek Venkatesh2, Suchetha kumari N3, Prajna Bhandary4

1-Vice Dean and Professor, department of conservative dentistry and endodontics, AB Shetty Memorial Institute of dental Sciences, NITTE deemed to be university, Mangaluru, India

2- Post graduate, department of conservative dentistry and endodontics, AB Shetty Memorial Institute of dental Sciences, NITTE deemed to be university, Mangaluru, India

3- Professor, department of biochemistry and principal scientific officer, central research laboratory, KS Hegde  medical academy , NITTE deemed to be university, Mangaluru, India

4- Research assistant, central research laboratory, KS Hegde Medical Academy, NITTE deemed to be university, Mangaluru, India

Abstract-

Background- Given that diabetes has been declared a pandemic of non-communicable diseases, a chair-side, non-invasive, less technique-sensitive, cost-effective approach that reduces discomfort, anxiety and fear among the general population and encouraging them to monitor the level of glucose on a regular basis should be considered.

Methods- All salivary samples were collected 2 hours after breakfast between 9-11am. Volunteers were asked to sit upright on the dental chair and 2ml of saliva were collected in sterile fluoridated container. The standard salivary glucose level were measured using O-Toluidine reagent method using double beam spectrophotometer.

Results- The study found out that the salivary glucose levels are measured more accurately using O-toluidine reagent method. 1-5ug/ml of glucose standard solution is used for standardising normal salivary glucose levels using O-Toluidine reagent method and measuring the optical density using double beam spectrophotometer.

Conclusions- The study concluded that the normal salivary glucose level were measured more accurately by O-toluidine reagent method using double beam spectrophotometer. Therefore, it can be concluded that the standard glucose solution needed for measuring normal glucose level in the blood and saliva is in the ratio of 1:200 -1:160.

Keywords– Standard glucose solution, Saliva, Salivary glucose level, O-Toluidine reagent, Double beam spectrophotometer.

Published
2022-06-23
How to Cite
Shanbhag, Pratheek, Mithra Hegde, Suchetha N, and Prajna Bhandary. 2022. “Standardizing the Collection and Measurement of Glucose in Saliva and Its Relationship With Blood Glucose Concentration”. Romanian Journal of Diabetes Nutrition and Metabolic Diseases 29 (2), 188-93. https://rjdnmd.org/index.php/RJDNMD/article/view/1114.