Nrf2 induction alleviates metabolic disorder and systemic inflammatory response in rats under a round-the-clock lighting and high-carbohydrate–lipid diet
Abstract
Background and aims: The aim of this work is to study the effect of Nrf2 inductor dimethyl fumarate (DMF) on metabolic disorder and systemic inflammatory response in rats exposed to round-the-clock lighting (RCL) and kept on the high-calorie carbohydrate-lipid diet (HCLD). Materials and methods: The experiment was performed on 21 Wistar white male rats. Control rats (Group 1) were fed standard control chow and kept on 12/12 hours light / dark cycle. Rats of the Group 2 were kept on a HCLD for 8 weeks and exposed to RCL, thus simulating metabolic syndrome development. The animals of the Group 3 received DMF in 10% dimethyl sulfoxide solution in a dose of 15 mg/kg intraperitoneally 3 times a week starting on the 30th day of the experiment. Results: DMF administered against the RCL and HCLD background lowered the serum glucose, insulin, cholesterol of very low density lipoproteins and triglycerides levels and elevated the cholesterol of high-density lipoproteins concentration compared with the findings of Group 2. Administering DMF under RCL and HCLD reduced serum tumour necrosis factor-alpha, С-reactive protein, and blood tiobarbituric acid-reacting compounds concentration compared to the results in Group 2. Conclusions: The induction of Nrf2 signalling system can be regarded as a potential target in the experimental therapy of metabolic syndrome, triggered by the disturbance of light / dark cycle and Western diet.
