THE EFFECT OF THYMOQUINONE TOWARD TOOTH DISORDER PREVENTION IN POSTNATAL RAT OFFSPRING BORN FROM HYPERGLYCEMIA PREGNANT RATS

Abstract

Background and aims: Hyperglycemia in pregnancy was caused by reduced insulin production that led to tooth germ growth disorder. Thymoquinone could increase insulin production through pancreatic β-cells regeneration. The purpose of this study was to determine the effect of thymoquinone to prevent tooth disorder in rat offspring born from hyperglycemia pregnant rat. Material and method: Wistar rat offspring used in this research taken from pregnant rat induced hyperglycemia with streptozotocin 40 mg/kgBW divided into four groups. Rat offspring observation had been done on 3^rd, 5^th, and 7^th day postnatal. The histological image of first maxillary molar tooth germ stained with Haematoxilin Eosin and Mallory’s Trichrome. The parameters of rat offspring were body weight, blood glucose levels, tooth growth stages, tooth dimension width, and tooth germ enamel matrix. Results: Thymoquinone group had the widest tooth germ dimension compare to other groups. All rat offspring tooth germs were at the apposition-calcification stage in matrix enamel (pre-enamel). The analysis showed that no statistical differences between thymoquinone group and metformin group (p>0.05). Conclusions: Thymoquinone has same function with metformin to prevent tooth disorder in rat offspring born from pregnant rat induced hyperglycemia.